They joined staff from the Trust and used grapple hooks, flung into the water, to drag litter and rubbish from the bed of the waterway back onto dry land.
A knife, tyres, bike frame, wheel clamp, scaffolding poles, and even a set of keys and a wine glass, were amongst a bumper haul.
The volunteers were from Gerald Eve, a local consulting firm.
Jack Newman, Trust volunteer leader, said: "We're really grateful to the volunteers for turning out and helping clean the canal. Grappling is a real art, so hopefully they had some fun too.
“London's canals are more popular than ever, and Little Venice is a real hub for the waterways in the city, so it's great to get some help to clean up what lies beneath the water line.
"We're getting more and more support from local volunteers to do our work, so if anyone is keen to get involved, do get in touch."