The Great British Bike Off will see members of the public join Canal & River Trust staff on a route from London to the Anderton Boat Lift.
Starting on the 25 September, the four day journey on back roads and suitable cycle paths will takeb in some scenic waterway sites, such as Hatton Locks and Standedge Tunnel.
All the money raised from The Great British Bike Off will go directly towards looking after the nation's canals and rivers.
Ruth Ruderham, head of fundraising at the Trust, says: “I want to thank all the participants for choosing to take part in this challenge in order to raise money for the waterways. I am sure they will have great fun along the way and come away knowing that their efforts have all been worthwhile.”
The event is supported by the Daily Express, who are sponsoring the cycling shirts and each participant will receive a water bottle sponsored by Irwin Mitchell.